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Basal levels of glutathione in the three cell lines were next examined, with the cisplatin-resistant BT16 cell lines having significantly higher levels than BT12 cells and G401 cells

The results suggest that multisite phosphorylation of the MTHFR enzyme is associated with SAM attachment to inhibit MTHFR activity in cells [23]

Effect of pH on H 2 O 2 Production by GSH Quantification of H 2 O 2 production by 10 and 30 mM GSH at its intrinsic pH (5.4 and 3.3, respectively) and at pH 7 0.2 (buffered using NaOH) in 1 PBS was analyzed using a Hydrogen Peroxide Assay Kit (Fluorometric-Near Infrared) (Abcam, Australia) complemented with a fluorescence plate reader (Tecan infinite M1000 pro)

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